产品概述 MTT Assay Kit ab211091 is an easy-to-use, non-radioactive, and high-throughput assay for measuring cell proliferation, cell viability and cytotoxicity.The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product.NB: MTT is also available as free molecule as ab146345 (Thiazolyl blue tetrazolium bromide).Viable cells with active metabolism convert MTT into formazan. Dead cells lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells.The MTT assay is a measure of the metabolic activity of the cells analyzed; the more metabolic activity in the sample, the higher the signal. This should be considered when interpreting the results of the assay.MTT assay protocol summary:- replace serum-containing media with serum-free media and MTT reagent in cell cultures- incubate for 3 hr at 37 C- add MTT solvent and incubate for 15 min- analyze with microplate reader Review our cell health assay guide to learn about our kits to perform acell viability assay, cytotoxicity assayorcell proliferation assay. An alternative product, MTS assay kit ab197010, uses a similar principle to this kit, but without the need for the MTT solvent step.How other researchers have used MTT Assay Kit ab211091This MTT assay kit has been used in publications with a variety of cell types, including:HUVEC cells1, U2OS cells and Saos2 cells2, human PBMCs3, rat primaryhepatic stellate cells4, DBTRG glioblastoma cells5, SKOV3 human ovarian cancer cells6, mouse vascular smooth muscle cells7, human iPSC-derived neuronal cultures8, primary bladder cancer cells9References: 1-De Felice F et al 2019, 2-Zhang X et al 2019, 3-Schotterl S et al 2018, 4-Huang W et al 2015, 5-Tang XJ et al 2016, 6-Zhu Q et al 2017, 7-Ma S et al 2018, 8-Rane A et al 2018, 9-Maj M et al 2018 Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. MTT assay kit ab211091 was used by an Abcam customer (see product review) to assess the cell viability of myeloma cells which have acquired resistance to bortezomib (a proteasome inhibitor used as a cancer therapeutic).Cells previously treated with 10 nM bortezomib for 1.5 months (RPMI8226-Bortezomib) had significantly improved viability, compared to the parental untreated cell line, when treated for 72 hours with varying concentrations of bortezomib. HeLa cells were grown in DMEM media supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were then serially diluted in a clear cell culture plate and incubated for 3 hours with MTT Reagent at 37 C. After incubation, cells were treated with MTT Solvent for 15 minutes at room temperature. Absorbance was measured at OD = 590 nm. Inset graph is an expanded segment of the assay data at lower cell number per well. 发表研究结果有使用 ab211091？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab211091 被引用在 58 文献中. Lu T et al. Circular RNA circCSNK1G3 induces HOXA10 signaling and promotes the growth and metastasis of lung adenocarcinoma cells through hsa-miR-143-3p sponging. Cell Oncol (Dordr) 44:297-310 (2021).PubMed: 33118120 Alamer A et al. Bismuth oxide nanoparticles induce oxidative stress and apoptosis in human breast cancer cells. Environ Sci Pollut Res Int 28:7379-7389 (2021).PubMed: 33030691 Lu W et al. Tetraspanin CD9 interacts with a-secretase to enhance its oncogenic function in pancreatic cancer. Am J Transl Res 12:5525-5537 (2020).PubMed: 33042435 Zhang B et al. Apigenin protects human melanocytes against oxidative damage by activation of the Nrf2 pathway. Cell Stress Chaperones 25:277-285 (2020).PubMed: 31953635 Yang C et al. Inflammation and DNA Methylation-Dependent Down-Regulation of miR-34b-5p Mediates c-MYC Expression and CRL4DCAF4 E3 Ligase Activity in Colitis-Associated Cancer. Am J Pathol 190:674-688 (2020).PubMed: 31972160 View all Publications for this product 1×105 cells exposed upon 10 nM bortezomib for 1.5 months in 100 µl media were seeded in each well of 96-well plates. The cells were then incubated with gradient dilutions of bortezomib at final concentrations of 0, 0.15625, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 nM 37°C in a total of 150 µl media in humidified incubator containing 5% CO2. After 72 hours of incubation, cell viability was examined by MTT assay. Experiments were carried out in quadruplicate, and the data were processed with GraphPad Prism. The results were shown as mean ± SD of three independent experiments.Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com